Ligand Exchange between Proteins
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چکیده
منابع مشابه
Ligand exchange between proteins. Exchange of biotin and biotin derivatives between avidin and streptavidin.
We have studied the structural elements that affect ligand exchange between the two high affinity biotin-binding proteins, egg white avidin and its bacterial analogue, streptavidin. For this purpose, we have developed a simple assay based on the antipodal behavior of the two proteins toward hydrolysis of biotinyl p-nitrophenyl ester (BNP). The assay provided the experimental basis for these stu...
متن کاملLigand Exchange Chromatography
of amino alcohols using Z-L-Glu-L-Pro or Z-L-Glu-D-Pro as chiral counter ions and Hypercarb as the solid phase. Chirality 9: 650}655. Knox JH and Jurand J (1982) Separation of optical isomers by zwitterion pair chromatography. Journal of Chromatography 234: 222}224. Knox JH and Ross P (1997) Carbon-based packing materials for liquid chromatography. Advances in Chromatography, 74}162. Petterson ...
متن کاملMechanistic Investigation of Ligand Exchange in Guanidinate Ligand Systems
Electronic Supplementary Information Mechanistic Investigation of Ligand Exchange in Guanidinate Ligand Systems Allison L. Brazeau, Gino A. DiLabio, Kevin A. Kreisel, Wesley Monillas, Glenn P. A. Yap and Seán T. Barry*
متن کامل[Ligand and isotope exchange in the system: serum proteins--zinc--polyaminopolycarbonic acids].
3 E . M Ü L L E R U. I . M Ü L L E R R O D L O F F , L i e b i g s A n n . C h e m . 517, 134 [1935]. 4 Bei der Aufnahme des in Abb. 2 wiedergegebenen Spektrums einer von + 6 0 °C abgeschreckten toluolischen Lösung von 1 wurde die Modulationsamplitude so gewählt, daß neben den Triplett-Signalen auch die HF-Struktur des //0-Signals sichtbar ist. 5 R. SCHMIDT U. H.-D. BRAUER, Angew. Chem. 83, 498...
متن کاملFluoride-Promoted Ligand Exchange in Diaryliodonium Salts.
Diaryliodonium salts are shown to undergo rapid, fluoride-promoted aryl exchange reactions at room temperature in acetonitrile. Aryl exchange is shown to be exquisitely sensitive to the concentration of fluoride ion in solution; fast exchange is observed as the fluoride concentration approaches a stoichiometric amount at 50 mM substrate concentration. The reaction is slowed, but not halted if b...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m202874200